Data Catalog | Emodnet Biology

Data Catalog

[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [105018]
Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes
Windal, I.; Van Wouwe, N.; Eppe, G.; Xhrouet, C.; Debacker, V.; Baeyens, W.F.J.; De Pauw, E.; Goeyens, L. (2005). Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes. Environ. Sci. Technol. 39(6): 1741-1748.
In: Environmental Science and Technology. American Chemical Society: Easton. ISSN 0013-936X; e-ISSN 1520-5851, more
Peer reviewed article  

Available in  Authors 
    VLIZ: Open Repository 103939 [ OMA ]


Authors  Top 
  • Windal, I.
  • Van Wouwe, N.
  • Eppe, G.
  • Xhrouet, C.
  • Debacker, V., more
  • Baeyens, W.F.J., more
  • De Pauw, E.
  • Goeyens, L., more

    Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene eXpression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes, mussels, starfishes, sea birds, and marine mammals). Good correlations are usually observed, but there are systematic differences between the results. Attempts are made to identify the origin of the discrepancy between the two methods.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors 


Website hosted and developed by VLIZ